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KMID : 0613820220320060430
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Journal of Life Science
2022 Volume.32 No. 6 p.430 ~ p.437
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A Study on the Cosmetic Materials of Abies nephrolepis MAX. Extracts with Whitening Activity
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Oh Min-Jeong
Yeom Hyeon-Ji
Chae Jung-Woo
Lee Jin-Young
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Abstract
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We conducted a study on the whitening activity of Abies nephrolepis MAX. extracts. The electron-donating ability of 70% ethanol extracts from A. nephrolepis MAX. Stem (AN-S) and A. nephrolepis MAX. Leaf (AN-L) were found to be 89.4% and 90.9% at 1,000 μg/ml concentration, respectively. Their ABTS+ radical scavenging abilities were found to be 88.8% and 96% 500 μg/ml concentration, respectively. Their tyrosinase inhibitory effects were found to be 48.5% and 31.1% at 500 μg/ml concentration, respectively. Cell survival rates measured in B16F10 were examined with the AN-S and AN-L extracts. Results showed cell viabilities of 98.3% and 94.4% at 500 μg/ml concentration, respectively. The protein and mRNA expression inhibitory effects of AN-S and AN-L extracts were measured by Western blotting and RT-PCR, respectively, at 25, 50 and 100 μg/ml concentrations. The results of western blotting showed that the AN-S extract at 100 μg/ml concentration decreased the TRP-1 protein expression rate by 46%. The results of Western blotting also showed that the AN-L extract at 100 μg/ml concentration decreased the MITF protein expression rate was by 54.6%. Consequently, in the case of MITF, TRP-1, TRP-2, and tyrosinase, the amount of mRNA expressed decreased as the concentration of the extracts increased. Our results suggested that A. nephrolepis MAX. extracts can be used as a functional cosmetic material by confirming that they can be used as a whitening material.
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KEYWORD
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Abies nephrolepis MAX., MITF, TRP-1, tyrosinase, whitening
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